Trabalho de Conclusão de Curso

Documento

Autoria

Luiz, Gabrielli Caroline Leal

Unidade da USP

Data de Apresentação

Orientador

Guimaraes, Elaine Aparecida Del Bel Belluz

Título em Inglês

Elucidating the role of the trigeminal complex on L-DOPA-induced orofacial dyskinesia in a rat model of Parkinson's disease: from muscular and nociceptive perspectives

Palavras-chave em Inglês

Parkinson's disease
L-DOPA-induced dyskinesia
Trigeminal system
Lateral pterygoid muscle
Astroglial reaction

Resumo em Inglês

Patients with Parkinson's Disease (PD) long-term treated with l-3,4- dihydroxyphenylalanine (L-DOPA) display some orofacial manifestations that include motor and non-motor symptoms affecting stomatognathic structures. Until now, the mechanism underlying this phenomenon has remained unclear. There is considerable evidence indicating the important role of the basal ganglia and the dopaminergic mechanisms in the regulation of orofacial movements and sensitivity. However, there is no evaluation of these influences on neuronal and glial activation of the trigeminal system. To investigate the modifications of the orofacial region in parkinsonism and LDOPA-induced dyskinesia and the neural mechanisms related to these modifications, we used the PD rat model produced by unilateral injection of 6-hydroxydopamine (6- OHDA) into the medial forebrain bundle, followed by L-DOPA chronic treatment. The abnormal involuntary movements, (characteristic of dyskinesia) and the sensorial (nociceptive) response, were determined. Lateral pterygoid muscles were removed for morphological and histologic analyses. The expression of FosB and glial markers (GFAP and OX-42) were analyzed in the trigeminal (motor and spinal) and facial nucleus. An increased hyperalgesic behavior was noted on 6-OHDA lesioned rats, as well as, on dyskinetic ones. Hemiparkinsonian rats showed an increase on myosin fibers on both lateral pterygoid muscles (right and left), while in the L-DOPA treated rats,There was a decrease in these fibers on contralateral to lesion side, the most affected side by dyskinesia in our model. There is also a bilateral increase in glycolytic metabolism and an inflammatory profile on these muscles of dyskinetic rats. We also investigated the expression of Fos-B and glial markers (GFAP and OX-42) in the motor and spinal trigeminal and facial nucleus. We found an increased expression of Fos B in the spinal nucleus of lesioned rats and on the motor and facial nucleus in L-DOPA-induced dyskinetic rats in the contralateral side to the lesion. About GFAP and OX-42, they were found increased in facial nucleus contralateral to lesion side. Our results suggest that spinal trigeminal nucleus activation may be associated to sensorial information on lesioned rats and that L-DOPA-induced dyskinesia, besides producing an orofacial sensorial impairment, promote a fatigue profile on lateral pterygoid muscle and that mainly the motor and facial nucleus are directly involved in these changes

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Data de Publicação

2020-02-07

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